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The mediator is incorporated in a transversal position across the membrane and permits ion transport Examples are substances with helical molecular structure, where the ion passes through the helix The selectivity is connected with the ratio of the helix radius to that of the ion (d) A membrane pore that permits hydrodynamic flux through the membrane No selectivity is involved here A number of substances have been discovered in the last thirty years with a macrocyclic structure (ie with ten or more ring members), polar ring interior and non-polar exterior These substances form complexes with univalent (sometimes divalent) cations, especially with alkali metal ions, with a stability that is very dependent on the individual ionic sort They mediate transport of ions through the lipid membranes of cells and cell organelles, whence the origin of the term ion-carrier (ionophore).

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They ion-specifically uncouple oxidative phosphorylation in mitochondria, which led to their discovery in the 1950s This property is also connected with their antibiotic action Furthermore, they produce a membrane potential on both thin lipid and thick membranes These substances include primarily depsipeptides (compounds whose structural units consist of alternating amino acid and ar-hydroxy acid units) Their best-known representative is the cyclic antibiotic, valinomycin, with a 36-membered ring [L-Lac-L-Val-D-Hy-i-Valac-D-Val]3, which was isolated from a culture of the microorganism, Streptomyces fulvissimus Figure 613 depicts the structure of free valinomycin and its complex with a potassium ion, the most important of the coordination compounds of valinomycin Complex formation between a metal ion and a macrocyclic ligand involves interaction between the ion, freed of its solvation shell, and dipoles inside the ligand cavity.

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The standard Gibbs energy for the formation of the complex, AG v, is given by the difference between the standard Gibbs.

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Fig. 6.13 Valinomycin structure in (A) a non-polar solvent (the 'bracelet' structure) and (B) its potassium complex. O = O, o = C, # = N (According to Yu. A. Ovchinnikov, V. I. Ivanov and M. M. Shkrob) energy for ion transfer from the vapour phase into the interior of the ligand in solution, AG _^V> and the standard Gibbs energy of ion solvation, AG j : AGJV = AGj_*v ~ AGs,j ~ (6.4.5)

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Zooming during shooting can be a visually stimulating sensation for your audiences, forcing them to get closer to the scene or subject as the action unfolds by editing out extraneous details. Too much of a good thing, however, can leave them feeling drained by excessive camera movement, swings, and zooms. Consider holding the camera with a very rm but gentle grip when shooting videos and try to avoid any quick, drastic movements in any direction, or better yet, use a tripod with a uid three-way pan/tilt head for smooth movements of the camera during shooting. Too much zooming in and out can be unsettling to an audience and give the impression you have no idea what is important in your clip. Be deliberate in your framing and compose your scenes just as you would with your favorite still images. One nal note of caution that may not be apparent when you rst begin shooting videos: My older brother Kevin had been shooting videos, mostly of his kids, for years before I started shooting digital videos, and his videos always entertained. My initial attempts were less than stellar, and the more I thought about it, the difference was in the audio not in the video. My voice was plainly obvious throughout most of my clips, yet he barely spoke in any of his; he just let the action unfold and didn t try to direct while shooting. In my videos, my voice was so overbearing! As I held the camera, the small on-board microphone was very close to my voice and picked it up over most other ambient sounds. Remember this the next time you re shooting videos, and let the action speak for itself.

As the quantities on the right-hand side are different functions of the ion radius, the Gibbs energy of complex formation also depends on this quantity, but not monotonously. The dependence of the Gibbs energy of solvation, e.g. for the alkali metal ions, is an increasing function of the ion radius, i.e. ions with a larger radius are more weakly solvated. In contrast, the interactions of the desolvated ions with the ligand cavity are approximately identical if the radius of the ion is less than that of the cavity. If, however, the ion

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