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Here, a glass capillary (a micropipette) with an opening of about 1 jum2 is pressed onto the surface of the cell and a very tight seal between the phospholipids of the membrane and the glass of the micropipette is formed (cf page 441) Because of the very low extraneous noise level, this patch-clamp method permits the measurement of picoampere currents in the millisecond range (Fig 621) Obviously, this course of events is formally similar to that observed with the gramicidin channel (page 448) but the mechanism of opening and closing is different In contrast with the gramicidin channel, the nerve cell channels are much larger and stable formations Usually they are glycoproteins, consisting of several subunits Their hydrophobic region is situated inside the membrane while the sugar units stretch out.



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All the networks in Figure 5.18 have a /27 network mask. This means that there are 30 hosts and two addresses reserved for the network and broadcast. (/27 means 24 bits for the first three octets plus 3 bits from the last octet, leaving 5 bits for host addressing. This means that there are 25 = 32 possible addresses, 30 for hosts and two for the network and broadcast.) Looking at this example, five of these networks can be assigned to each of the router interfaces. However, the connection between the routers and that between one of the routers and the Internet only require two host addresses for their respective interfaces.





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Ion channels of excitable cells consist of a narrow pore, of a gate that opens and closes the access to the pore, and of a sensor that reacts to the stimuli from outside and issues instructions to the gate The outer stimuli are either a potential change or binding of a specific compound to the sensor The nerve axon sodium channel was studied in detail (in fact, as shown by the power spectrum analysis, there are two sorts of this channel: one with fast opening and slow inactivation and the other with opposite properties) It is a glycoprotein consisting of three subunits (Fig 622), the largest (mol wt 35 x 105) with a pore inside and two smaller ones (molwt 35 x 104 and 33 x 104) The attenuation in the orifice of the pore is a kind of a filter.

Figure 5.18 There are five networks that need IP address assignments. We need to create subnets from a common IP network address for all these networks.

10 20 30

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Using ash for action/sports photography is often inadvisable. Sometimes you are so far away from the action that your ash won t be effective, or you may be in a situation where ash is not allowed because it s distracting to the participants. In these cases, just make sure you use a fast-enough shutter speed to freeze the motion. Remember to use as fast a shutter speed as you can, depending on your ISO setting. You can also use a wide aperture in Manual or dial up your ISO setting to be sure you get a workable shutter speed.

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subnets have been assigned to the network randomly, as shown in Figure 5.19. Each of the subnets supports 30 hosts. However, the link between the routers is a point-topoint link and only requires two host addresses. The broadcast networks attached to a switch could require 60 hosts each but are limited to 30 hosts. How do we rectify this situation

E E 60 70 80-

Figure 5.19 Using 27 bits for the subnet mask, we could create eight subnets that can each support 30 hosts. However, 30 hosts may be too few for some subnets, and too many for others.

lnmnnnni:

192.168.10.0/27 192.168.10.96/27

For many photographers, the need to shoot at fast shutter speeds produces incredible images of moderately paced action successfully The PowerShot G11 responds quickly to your touch, and AF is adequately fast to capture that shot at the peak of the action In addition, when you shoot in Continuous mode, you ll be better positioned to capture the decisive moment of the play over several frames Top-of-the-line pro cameras are renowned for their faster frames-per-second rates, and the G11 res approximately 24 frames per second, which is still ne for lots of sporting events By cramming more images into that 1-second time frame, the odds are higher you ll yield far more keepers of the peak action For anyone who appreciates fast action, shooting sports can be one of the more exciting and rewarding genres of photography.

Fig. 6.21 Joint application of patch-clamp and voltageclamp methods to the study of a single potassium channel present in the membrane of a spinal-cord neuron cultivated in the tissue culture. The values indicated before each curve are potential differences imposed on the membrane. The ion channel is either closed (C) or open (O). (A simplified drawing according to B. Hille)

192.168.10.128/27 192.168.10.32/27 192.168.10.64/27

Fig. 6.22 A function model of the sodium channel. P denotes protein, S the potential sensitive sensor and H the gate. The negative sign marks the carboxylate group where the guanidine group of tetrodotoxin can be attached. (According to B. Hille)

The solution is to assign different subnet masks to the network (this is very typical in real networks). Use a different mask (e.g., use /26 for 192.168.10.0/24) to generate subnetworks such as: 192.168.10.0/26 192.168.10.64/26 192.168.10.128/26 192.168.10.192/26 (all with 62 hosts each).

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