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Lallier, Beam clean-up of a multimode Yb-doped ber ampli er with an infrared sensitive Rh:BaTiO3 crystal, Technical Digest CLEO 2001, Paper CTuQ7, Baltimore, 6 10 May 2001 67 A Tomita, Phase conjugation using gain saturation of a Nd:YAG laser, Appl Phys Lett 34, 463 464 (1979) 68 P Sillard, A Brignon, and J P Huignard, Loop resonators with self-pumped phase conjugate mirrors in solid-state saturable ampli ers, J Opt Soc Am B 14, 2049 2058 (1997) 69 K S Syed, G J Crots, and M J Damzen, Transient modelling of a self-starting holographic laser oscillator, Opt Commun 146, 181 185 (1998) 70 P Aubourg, J P Huignard, M Hareng, and R A Mullen, Liquid crystal light valve using bulk monocrystalline Bi12SiO20 as the photoconductive material, Appl Opt 21, 3706 3712 (1982) 71 J C Chanteloup, B Loiseaux, J P Huignard, and H.



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Baldis, Detection and correction of the spatial phase of ultrashort laser pulses using an optically addressed light valve, Technical Digest, CLEO 97, Paper CThD8, Baltimore 1997 72 S A Chetkin and G V Vdovin, Deformable mirror correction of a thermal lens induced in the active rod of a solid state laser, Opt Commun 100, 159 165 (1993) 73 Special Issue Opt Eng 36 (1997) See also M A Vorontsov, G W Carhart, D V Pruidze, J C Ricklin, and D G Voelz, Adaptative imaging system for phase-distorded extended source and multiple-distance objects, Appl Opt 36, 3319 3328 (1997)..





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Protein kinases and phosphatases (PPs) combine to play a major role in the regulation of cell function by, respectively, phosphorylating and dephosphorylating proteins Three groups of phosphatases (which hydrolyze phosphate groups from phospho-amino acids) have been described in eukaryotes differing on substrate speci city (ie; protein serine/threonine, tyrosine and serine/threonine/tyrosine phosphatases) One of the protein serine/threonine phosphatase enzymes is found in two distinct gene products; PP2C and Five cDNA clones for the latter have been identi ed which differ only in their C-terminal sequence and which also show tissue-speci c expression Crystallographic data and point mutations, however, identi ed a possible N-terminal catalytic domain It is thought that PP2C isoenzymes may have distinct physiological roles in each tissue but the relative importance of the N- and C-terminal domains to this are unclear.

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he topics in the previous chapters have worked with two axes, X and Y. In this chapter, I add the Z axis. When you have a drawing with 3D objects, you can view it from any angle. The view that you ve been using in 2D drawings is like looking at a house from the top, which you could call a plan view or a floor plan. From this view, even a 3D drawing looks two-dimensional. But when you look at a 3D drawing from an angle, you can see that there s more to it than meets the eye. Figure 21-1 shows the plan view of an office building. Figure 21-2 shows the same drawing viewed in a perspective view from the front.

This question was probed by selectively deleting parts of the PP2C sequence from both the N- and the C-terminus (A) These mutants were puri ed from a GST fusion expression system ( 2; The residues Gly - Ser- Pro originate from the GST portion of the fusion) and analysed by western blotting (B) and catalytic assay of dephosphorylation (C) The western blot (B) carried out with antiserum to PP2C showed a single band except in the case of C(313 390) and C(291 390) where two bands were observed The blot is an important part of the experiment as it allows con rmation of deletion of large parts of the N- and C-terminal domains as evidenced by the greater electrophoretic mobility of mutants Moreover, the presence of two bands for C(313 390) and C(291 390) allowed identi cation of inappropriate thrombin digestion of the GST-PP2C fusion (Figure 219).

A function very useful for enhancing the display image is histogram modi cation. A histogram of the original image is rst obtained and then modi ed by rescaling each pixel value in the original image. The new enhanced image that is formed will show the desired modi cation.

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