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electrostatic repulsion that opposes a strong penalty to the formation of loops shorter or closer than the Debye length Exposure to UV irradiation triggers the detachment of the bound micelles (or proteins) (Pouliquen and Tribet, 2006; Pouliquen et al, 2002) Likewise, we cannot detect signi cant sensitivity to the presence of nanoparticles of the absorption spectrum of the predominantly cis polymer (not shown) However, to obtain more accurate determinations of the degree of light-triggered release, the average number of bound micelles (or protein) per chain was measured Chromatographic methods under continuous injection mode, in this case, capillary electrophoresis, enable one to separate minute amount of free particles with no marked perturbation of the binding equilibrium [the method is also called frontal analysis; see Gao et al 1997] Representative binding isotherms in Fig 77 obviously point to UV-triggered releases.



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The degree of photodissociation depends, however, on the composition of the complexes In the case of the protein bovine serum albumin, we measured an increasing degree of dissociation with the decreasing number of protein per chain (Fig 77b and Pouliquen and Tribet, 2006) Saturated chains with typically one bound protein per one to three azobenzene groups do not respond to light, which points to the lack of speci city of the protein toward the isomerization state of the azobenzene Nevertheless, chains with less than 2 4 g/g of bound protein (ie, less than three proteins per 1000 monomers and 10 30 azobenzene groups per bound protein) release up to 90% of their protein content upon exposure to blue light In contrast, surfactants such as Triton X100 (Fig 77a) and C12E4 (Khoukh et al.

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The principle of operation of the EHA is given in 1. The following is a selection of EHA applications on existing programmes or demonstrators: EHAs for A380 ailerons (4) and elevator (4) EHAs for F-35/JSF primary ight control surfaces (5) A380 rudder EBHAs (4) and spoiler EBHAs (4)

, 2007) do not photodissociate at low degree of loading of the micelles in the chains (close to the critical micellar concentration, where B20 40 azobenzene bind to 100 surfactants) The photoresponse increases regularly with increase in the loading of surfactant and accordingly decrease in the amount of bound azo per micelle A tentative interpretation is proposed that in both cases, an optimal number of ca 10 20 bound azobenzene per bound nanoparticle must be reached for optimal responses to be reached The following arguments present the basic reasons for an optimal azo/particle ratio, and the expected polymer-related ampli cation of af nity Binding of an isolated azobenzene on a single nanoparticle should a priori be described by using a conventional equilibrium constant as for 1:1 complexation (we will call this incipient binding the Benessi Hildebrand regime).

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