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Obstacle detection may be a scenario example of the CORTEX Car Control application. In order to avoid colliding with both obstacles on the road and other cars, it should be able to detect its position and the positions of other cars, and take action upon this information. If a car detects an obstacle ahead, it should rapidly apply an emergency stop and disseminate an emergency signal. Cars receiving an emergency stop signal from a car ahead should also apply an emergency stop themselves. The picturized scenario is given in Figure 2.7. 2.7.3.7. System requirements Autonomy: as stated before, this scenario aims at searching for possible ways of providing traf c control without human intervention. Cars need to make autonomous decisions such as deciding when to brake or change the car speed. Decision-making takes place based on current and past context information. A control logic engine is necessary to ful ll such autonomous behavior. Cooperation: in order to avoid of human-based traf c control, some degree of cooperation between cars is required. Each car noti es other cars about the actions it takes by dissemination of events. The events are propagated to all cars in its proximity. Event dissemination should be as real-time as possible. For instance, a car suddenly slows down and the car is followed by many other cars. The



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by another pair of subscribers. During the period of occupancy of a speech path endto-end, we must know that this particular path is busy (i.e., it is occupied) so no other call attempt can seize it.

agement of groundwaters unless their neighbors do so too. Some of the potential problems associated with transboundary groundwaters include (Figure 11.1):

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Collagenase (See Section 2.1.1(v)) Petri dishes, non-tissue culture grade, 10 cm Protocol (a) Detach the ES cells with collagenase as previously described (See Protocol 3.3). (b) Resuspend the cells in EB medium and add to non-tissue culture-grade polystyrene dishes. (c) Usually the cells are seeded so that one 10-cm ES dish is split into three 10-cm EB dishes (or similar). (d) Put the dishes in the CO2 incubator. After one or two days, the cells typically form clusters that range in size from 50 to 1000 m.

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of multiplying the number by itself compared with being given the result and asked to nd the square root. The number of steps required to do so, is much greater than for the squaring operation. Another, more relevant operation, is that of factorising products of prime numbers . We remind ourselves that prime numbers are whole numbers that cannot be factorised into the product of a number of smaller whole numbers. 1, 2, 3, 5, 7 are primes, but not 4 ( 2 2), 6 ( 2 3) or 9 ( 3 3). It is known there are an in nite number of primes, about 10 150 of them with 512 bits or less, but there is no known formula for proving, in general, that a number is or is not a prime, that is signi cantly quicker than trial or error. The time taken to do this increases very rapidly with the size of the number. A similar problem is that of factorising the product of two prime numbers . (i.e. nding p and q, given only p time q.) It is easy to multiply two prime numbers together, but dif cult to reverse the process.

As stated before, heat treatment s rst aim is to destroy pathogens, spoilage microorganisms, and enzymes. Theoretical considerations for microbial destruction are also valid for enzyme inactivation (Dziezak 1991). The main criteria for thermal destruction are: (1) all spores and viable cells able to grow and produce toxins must be eliminated, taking as a calculation basis C. botulinum, the most dangerous microorganism from the public health point of view; (2) spoilage microorganisms must be reduced to a limit that ensures food quality for a given time. From a commercial point of view, a food can be considered sterile if it is free from Bacillus stearothermophilus or Clostridium perfringens. In general, the strict anaerobe C. botulinum is taken as the target microorganism due to its pathogenicity; however, other target microorganisms are B. stearothermophilus, B. thermoacidurans, B. macerans, and B. polymyxa (Guerrero Legarreta 2001), in addition to speci c pathogens most likely to colonize a speci c food. In the case of raw poultry meat and poultry products, these are C. prefringens, Salmonella spp., Staphylococcus spp., and Campylobacter spp. Microbial inactivation calculations are based on how long the food shelf life must be extended. Sporulated thermophiles must also be considered if the food will be stored at high

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