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35 kDa Only 13% of these proteins exceed the GroEL mass cut-off (55 kDa) In eukaryotes, the average protein size is larger (53 kDa) and 38% of yeast s 5800 proteins exceed the GroEl cut-off Coupled with the fact that eukaryote protein synthesis is signi cantly slower than bacteria, this suggests that post-translational chaperonin activity may not be as important in these organisms A possible reason for this may be a preference for cotranslational domain-wise folding in eukaryotes (ie folding of individual domains while translation of other domains is still in progress) compared to a preference for post-translational folding in prokaryotes Indeed it has been suggested that, during evolution, domains which had a predisposition to fold cotranslationally may have been selected and this may explain why eukaryote proteins heterologously expressed in bacteria so often aggregate to form inclusion bodies.



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Rules for determining R99 attributes from R97/98 attributes [9]. Derived from R97/98 attribute Value 1, 2, 3 4 1 2 3 1, 2 3 4, 5 1, 2, 3, 4 5 1, 2, 3, 4 5 1 2 3 4 5 6 7 8 9 1 2 3 Yes No Name Delay class Delay class





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Chaperones make use of ATP-hydrolysis coupled to allosteric structural changes to promote protein folding in cells But many of the physicochemical forces driving folding are expected to be largely independent of chaperones and these proteins may merely provide a supportive microenvironment where these forces can operate Two main models for the contribution of chaperones to folding of slow-folding proteins have been proposed The An nsen cage model suggests that intermolecular aggregation could provide a limit to folding and that the main role of chaperones is to avoid this An alternative view is the iterative annealing model which proposes that the rate-limiting step in slow folding may be the intramolecular formation of incorrectly folded structures and that the role of chaperones is to unfold such polypeptides and give them an opportunity to fold correctly.

These two models are not mutually exclusive and it is possible that chaperones contribute to the folding of individual proteins in different ways depending on the particular ratelimiting step in their folding pathways..

PACS and Imaging Informatics, Second Edition, by H. K. Huang Copyright 2010 John Wiley & Sons, Inc. 927

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THREE-DIMENSIONAL STRUCTURE DETERMINATION OF MACROMOLECULES Vrielink, A. and Sampson, N. (2003) Sub-Angstrom resolution enzyme X-ray structures: Is seeing believing Current Opinion in Structural Biology, 13, 709 15. A review emphasizing how high-resolution structural data contribute to our understanding of enzyme mechanism.

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Obese people often describe atypical behaviours and attitudes related to eating and weight, such as binge eating, body image disturbance and a preoccupation with weight, shape and eating. These problems may contribute to the development and/ or maintenance of obesity, and in some cases, symptoms are so signi cant that an eating disorder is clinically diagnosable. The most common eating disorders observed in obese individuals are Binge Eating Disorder (BED) and Night Eating Syndrome (NES). As these disorders may signi cantly affect outcome, adequate assessment and treatment of the full range of eating disorders is critical in the management of obese patients. This chapter describes the features of disordered eating that are common in obesity, as well as the psychopathology associated with eating disorders in obese individuals. It concludes with practical guidelines for identifying, assessing and treating obese individuals with disordered eating.

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