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buy-in from the managers and supervisors who will be implementing projects identified within your Strategic Initiatives Your teams should have diverse people who are knowledgeable about existing projects and project management Although these meetings usually involve eight to 16 people, they can sometimes involve as many as 40 people To follow the process outlined below, you will want the teams to meet by Strategic Theme If there are a lot of initiatives in a Strategic Theme, you may want to limit each meeting to a few high-order initiatives These large-group meetings can last from 3 hours to all day 1 The Theme Team leaders guide the group through building its own case for change This interactive process enables the group to build its own case for change that parallels the ideas and process used with the executive team.



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and, hence, (37.11) which shows, as desired, that knowledge of 6Pi-l is also sufficient for the time-update of the conversion factor.





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The SONET ADM multiplexes one or more DS-n signals into the SONET OC-N channel. An ADM can be con gured for either the add drop or terminal mode. In the ADM mode, it can operate when the low-speed DS1 signals terminating at the SONET derive timing from the same or equivalent source as (SONET) (i.e., synchronous) but do not derive timing from asynchronous sources. Figure 17.14 is an example of an ADM con gured in the add drop mode with DS1 and OC-N interfaces. A SONET ADM interfaces with two full-duplex OC-N signals and one or more full-duplex DS1 signals. It may optionally provide low speed DS1C, DS2, DS3, or OC-M (M N) interfaces. There are nonpath-terminating information payloads from each incoming OC-N signal, which are passed the SONET ADM and transmitted by the OC-N interface at the other side. Timing for transmitted OC-N is derived from either an external synchronization source, an incoming OC-N signal, from each incoming OC-N signals in each direction (called through-timing), or from its local clock, depending on the network application. Each DS1 interface reads data from an incoming OC-N and inserts data into an outgoing OC-N bit stream as required. Figure 17.14 also shows a synchronization interface for local switch application with external timing and an operations interface module (OIM) that provides local technician orderwire, local alarm, and an interface to remote

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Reagents and Materials Nonsterile Cell lysis solution: Triton X-100, 0.2% (v/v) + 5 mM magnesium chloride in UPW Alkaline phosphatase (ALP) Kit Sodium hydroxide solution, 0.2 M Paper towel Razor blade Eppendorf tubes Microcentrifuge tubes with screw cap, 2 ml Steel balls Para lm Analytical balance MiniBeadBeater Microcentrifuge Microtitration plate Microplate reader Waterbath Protocol Note: Work on ice all times unless otherwise stated. A. Scaffolds (a) Grow MSCs on scaffolds in spinner asks as described in Protocol 13.9. (b) Dry scaffolds on a clean paper towel for 3 min on both sides. (c) Measure wet weight of the scaffolds. Eventually cut the scaffolds into halves and measure the wet weight of halves. (d) Put each scaffold into a labeled microcentrifuge tube. (e) Add 2 steel balls and 1 ml cell lysis solution per tube. (f) Close tube rmly and wrap with Para lm. (g) Disintegrate scaffold by using a MinibeadBeater 3 times at 25,000 rpm for 10 s. Place on ice between cycles for cooling. (h) Transfer the content of the tube into a clean labeled tube without transferring the steel balls. (i) Centrifuge at 300 g for 10 min at 4 C. (j) Transfer the supernate into a clean, labeled tube. Avoid destruction of the pellet. (k) Run ALP assay immediately. B. Cell pellets (a) Grow MSCs in pellet culture according to Protocol 13.7 or 13.8.

Pages in a PDF file can be renumbered using the Page Numbering dialog box that you access with a simple menu command. Open the Pages panel and open a context menu on a page thumbnail. From the menu options, select Number Pages. The Page Numbering dialog box opens, as shown in Figure 16.21.

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