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There are between 35 and 45 seeds (or beans or cotyledons) encased in a mucilaginous pulp known as the endocarp and composed of sugars (mainly sucrose), pectins, polysaccharides, proteins, organic acids and salts (Table 161) The plant contains alkaloids, notably the methylxanthines theobromine (1 2% of the dry weight) and caffeine (0 2%) (Fig 162) The former affords bitterness to cocoa The embryo of the seed comprises two folded cotyledons that are covered with a rudimentary endosperm It is these cotyledons that are used for making cocoa and chocolate (Fig 163) The ripe pods are harvested and their husks broken using sharp objects or wooden billets The wet beans are removed from the husk and heaped (50 80 cm deep) on the ground or in boxes (100 cm deep) to allow sweatings to drain from the bottom.

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The beans are covered mainly with banana leaves, and left for 5 7 days with one or more turnings to allow for a more even fermentation The temperature will rise to around 50 C and must be maintained below 60 C to avoid over-fermentation and excessive growth of fungi During fermentation, the pulp becomes infected with diverse microorganisms from the environment At the start of fermentation, the low pH and high sugar in the surrounding pulp favour anaerobic fermentation by yeasts and also the growth of lactic acid bacteria The ethanol produced represents a substrate for the acetic acid bacteria, which predominate when the sugars are exhausted Pectinolytic activity is supplied by Kluyveromyces marxianus, but Saccharomyces, Torulopsis and Candida are other yeasts that have signi cant roles to play The pectinolysis leads to the draining of the pulp off the beans as sweatings .

FIGURE 5.11 Click the color swatch for the Grid line color to choose from a selection of preset colors, or select Other Color to open the system color palette.

CONCLUDING COMMENTS As in other areas of service delivery, school psychologists can most likely avoid ethical-legal dilemmas in research by maintaining an up-to-date knowledge of relevant guidelines, careful planning of proposed research activities, and seeking consultation and advice from others when questions arise. School psychologists conducting research need to be knowledgeable of the organization and methodology of the school and to work within the organizational framework, taking care to build and maintain good public relations within and outside of the school community during all phases of a research project.

This allows air into the spaces between the beans and so, late in fermentation, aerophiles develop, including Bacillus, as well as lamentous fungi, such as Aspergillus fumigatus, Penicillium and Mucor spp..

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If the patient was pregnant and described multiple small raised purplish nodules affecting her anogenital area, what condition would you suspect and what treatment would you recommend

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