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24. An Hfr strain that is a b c d e is mated with an F strain that is a b c d e . The mating is interrupted every ve minutes, and the genotypes of the F recombinants are determined.The results appear following. (A plus indicates appearance; a minus the lack of the locus.) Draw a map of the chromosome and indicate the position of the F factor, the direction of transfer, and the minutes between genes.

0 = 0 =

Figure P840, R must maintain the Zener diode current within its speci ed limits for all values of the source voltage, load current, and Zener diode voltage If: Vz = 12 V 10% iz min = 325 mA VS = 25 15 V IL = 315 215 mA determine the minimum and maximum values of R which can be used rz = 9 iz max = 80 mA

26. De ne prophage, lysate, lysogeny, and temperate phage. 27. Outline an experiment to demonstrate that two phages do not undergo recombination until a bacterium is infected simultaneously with both. 28. Doermann (1953, Cold Spr. Harb. Symp. Quant. Biol. 18:3) mapped three loci of phage T4: minute, rapid lysis, and turbid. He infected E. coli cells with both the triple mutant (m r tu) and the wild-type (m r tu ) and obtained the following data:

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circuit shown in Figure P840 is a 1N5231B The source voltage is obtained from a DC power supply It has a DC and a ripple component: vS = VS + Vr where: VS = 20 V R = 220 Vz = 51 V iz min = 10 mA Determine the maximum rated current the diode can handle without exceeding its power limitation

+ V _ S _

m r tu 3,467 m r tu 474 m r tu 162 m r tu 853 m r tu 965 m r tu 172 m r tu 520 m r tu 3,729

Figure P840, the Zener diode is a 1N4740A Vr = 250 mV IL = 65 mA rz = 17 VL = 51 V PRated = 05 W Vz = 10 V 5% Prated = 1 W VS = 14 2 V rz = 7 R = 198 iz min = 10 mA iz max = 91 mA

25. A bacterial strain that is lys his val is used as a donor, and lys his val as the recipient. Initial transformants are isolated on minimal medium histidine valine. a. What genotypes will grow on this medium b. These colonies are replicated to minimal medium histidine, and 75% of the original colonies grow. What genotypes will grow on this medium c. The original colonies are also replicated to minimal medium valine, and 6% of the colonies grow. What genotypes will grow on this medium d. Finally, the original colonies are replicated to minimal medium. No colonies grow. From this information, what genotypes will grow on minimal medium histidine and on minimal medium valine e. Based on this information, which gene is closer to lys f. The original transformation is repeated, but the original plating is on minimal medium lysine histidine. Fifty colonies appear. These colonies are replicated to determine their genotypes, with these results:

Determine the minimum and maximum load current for which the diode current remains within its speci ed values

dc = R ( dc + Yc ) R ( dc + Yc ) 1 dc + Yc and choosing the physically correct root that corresponds to the minus sign, we have the process limit

Figure P840, the Zener diode is a 1N963 Determine the minimum and maximum load current for which the diode current remains within its speci ed values

What is the linkage relationship among these loci In your answer include gene order, relative distance, and coef cient of coincidence. 29. Wild-type phage T4 (r ) produce small, turbid plaques, whereas rII mutants produce large, clear plaques. Four rII mutants (a d ) are crossed. (Assume, for the purposes of this problem, that a d are four closely linked loci. The actual structure of the rII region is presented in chapter 12. Here, assume that a b means a b c d a b c d .) These percentages of wild-type plaques are obtained in crosses:

+ RL VL _

846 In the simple voltage regulator circuit shown in 841 The 1N963 Zener diode in the simple voltage

Deduce a genetic map of these four mutants. 30. A phage cross is performed between a b c and a b c phage. Based on these results, derive a complete map:

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