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P825 the diodes are 1N4001 with a rated peak reverse voltage (also called peak inverse voltage) of 25 V They are fabricated from silicon n = 005883 C = 80 F RL = 1 k Vline = 170 cos(377t)V a Determine the actual peak reverse voltage across each diode b Explain why these diodes are or are not suitable for the speci cations given

(44)

shown in Figure P825 are silicon If: IL = 600 mA Vr = 8% = 4 V Vline = 170 cos( t) V = 377 rad/s VL = 50 V

the rst or third positions for any of these codons still codes a hydrophobic amino acid. Both of the two negatively charged amino acids, aspartic acid and glutamic acid, have codons that start with GA.All of the aromatic amino acids phenylalanine, tyrosine, and tryptophan (see g. 11.1) have codons that begin with uracil. Such patterns minimize the negative effects of mutation. This chapter completes the discussion of the mechanics of gene expression.The next chapter deals with recombinant DNA technology, followed by several chapters concerned with the control of gene expression in both prokaryotes and eukaryotes.

shown in Figure P825 are silicon If: IL = 5 mA VL = 10 V = 377 rad/s Vr = 20% = 2 V Vline = 170 cos( t) V

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STUDY OBJECTIVE 1: To study the mechanism of protein biosynthesis, in which organisms, using the information in DNA, string together amino acids to form proteins 281 303 A charged transfer RNA has an anticodon at one end and a speci c amino acid at the other endThe transfer RNAs are charged with the proper amino acid by aminoacyl-tRNA synthetase enzymes that incorporate the energy of ATP into amino acid-tRNA bonds Hence, no additional source of energy is needed during peptide bond formation During protein synthesis, the translation apparatus at the ribosome recognizes the transfer RNAThrough complementarity, the anticodon pairs with a messenger RNA codon An initiation complex forms at the start of translation In prokaryotes,this complex consists of the messenger RNA,the 30S subunit of the ribosome, the initiator transfer RNA with N-formyl methionine (fMet-tRNAfMet), and the initiation factors IF1,IF2,and IF3.

P825: n = 01 C = 80 F RL = 1 k Vline = 170 cos(377t)V The diodes are 1N914 switching diodes (but used here

8

The 50S ribosomal subunit is then added and A and P sites form in the resulting 70S ribosome The charged N-formyl methionine transfer RNA is in the P site A GTP is hydrolyzed, and the initiation factors are released A transfer RNA enters the A site, which requires the involvement of elongation factors EF-Ts and EF-Tu (in E coli) At least one GTP hydrolysis releases the elongation factor, EFTu, which had originally brought the charged transfer RNA to the ribosome Peptidyl transferase, which appears to be a ribozymic component of the 50S ribosomal subunit, transfers the amino acid from the transfer RNA in the P site to the amino end of the amino acid on the transfer RNA in the A site With the help of elongation factor G (EF-G), the ribosome translocates in relation to the messenger RNA.

The straight line can be defined by Y = mX + C, where m is the slope and C is the intercept Putting equation 44 in terms of a straight line, we get

830 In the circuit shown in Figure 815:

vs1 (t) = vs2 (t) = Vs0 cos( t)

The depleted transfer RNA is moved from the P site to the E site, where it is released;the transfer RNA with the growing peptide is moved into the P site EF-G is then released Elongation and translocation continue until a nonsense codon enters the A site With the aid of the release factors RF1 and RF2, the protein is released, and the messenger RNAribosome complex dissociates Eukaryotes have slightly more complex processes involving several more proteins Proteins pass through membranes with the help of a signal peptide synthesized at their N-terminal endsProteins fold into their nal,functional con gurations with the help of molecular chaperones, proteins that aid the folding process Many antibiotics interfere with translation in prokaryotes Puromycin, streptomycin, tetracycline, and chloramphenicol all act at the ribosome Studying the mode of action of these antibiotics has provided insights into the mechanism of the translation process.

The diodes are silicon If the power rating of one of the diodes is exceeded and it burns out or opens, determine the new values of the DC output or load voltage and the ripple voltage

STUDY OBJECTIVE 2: To examine the genetic code.

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