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Guardans, R & BS Gimeno (1994) Long distance transportation of atmospheric pollutants and its effects on ecosystems Microbiology, 10, 145 158 Guillette, JL, DA Crain, AA Rooney & DB Pickford (1995) Organization versus activation: The role of endocrine-disrupting contaminants (EDCs) during embryonic development in wildlife Environmental Health Perspectives, 103 (Suppl 7), 157 164 Guttman, SI (1994) Population genetic structure and ecotoxicology Environmental Health Perspectives, 102 (12), 97 100 Hansen, PD, H von Westernhagen & H Rosenthal (1985) Chlorinated hydrocarbons and hatching success in baltic herring spring spawners Marine Environmental Research, 15, 59 76 Harmon, MA, MF Boehm, RA Heyman & DJ Mangelsdorf (1995) Activation of mammalian retinoid X receptors by the insect growth regulator methoprene Proceedings of the National Academy of Sciences of the USA, 92, 6157 6160 Harries, JE, DA Sheahan, S.



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(o) Open skin with scissors and create subcutaneous pockets with blunt forceps. (p) Insert cubes, up to 12 per mouse. (q) Close skin with staples. (r) Wipe ear with alcohol and make ear notch for identi cation. (s) Return mouse to cage, and repeat the process with as many mice as needed. (t) When nished, return cage to its original location. Wipe hood surface with water. Wipe any carts you used with Clidox, then with water. (u) Turn off hood.





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the deposited compounds, as well as by the thermal conditions of smoking In electrostatic smoking, the rapidly formed thick, sticky coat of smoke components is initially not xed to the meat surface and can be easily removed (Fig 121) However, during subsequent heating to the desired temperature, rapid penetration of the smoke components into the mass of the meat takes place Most phenols accumulate on the skin, on the sausage casing, and under the surface at about a 6 mm deep layer, especially in the fatty tissue However, in some products, even as much as 60% of the total mass of phenols can diffuse into deeper layers Carbonyl compounds and acids are rather equally distributed throughout the mass of some smoked meat products The smoke compounds accumulated in the meat cannot be 100% recovered by extraction, since many of them interact chemically with the meat components.

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Jobling, P Matthiessen, P Neall, E Routledge, R Rycroft, JP Sumpter & T Tylor (1996) A survey of estrogenic activity in United Kingdom inland waters Environmental Toxicology and Chemistry, 15, 1993 2002 Harries, JE, DA Sheahan, S Jobling, P Matthiessen, P Neall, JP Sumpter, T Tyler & N Zaman (1997) Estrogenic activity in ve United Kingdom rivers detected by measurement of vitellogenesis in caged male trout Environmental Toxicology and Chemistry, 16, 534 542 Hashimoto, S, H Bessho, A Hara, M Nakamura, T Iguchi, & K Fujita (2000) Elevated serum vitellogenin levels and gonadal abnormalities in wild male ounder (Pleuronectes yokohamae) from Tokyo Bay, Japan Marine Environmental Research, 49, 37 53 Heppell, SA, ND Denslow, LC Folmar & CV Sullivan (1995) Universal assay of vitellogenin as a biomarker for environmental estrogens Environmental Health Perspectives, 103 (Suppl 7), 9 15.

The proteins and lipids of meat contain various reactive groups, which in appropriate conditions, especially at high temperature, can react with phenols, carbonyl compounds, and acids of the smoke The most reactive are the SH, NH2 , and NH C(=NH)NH2 groups of amino acid residues in proteins and of nonprotein nitrogen compounds, as well as the polyenoic fatty acids and different reactive oxygen species and oxidation products like O2 , OH, RO , ROO , and ROOH Smoke compounds may react with amino acids in meat products, leading to a signi cant decrease in the contents of amino acids in acid hydrolysates (Seuss 1986) Coniferaldehyde and sinapaldehyde present in the smoke condensates were not recovered from the smoked meats in the experiments of Lustre and Issenberg (1970), possibly due to their interactions with the meat proteins.

Herman, RL & HL Kincaid (1988) Pathological effects of orally administered estradiol to rainbow trout Aquaculture, 72, 165 172 Hesthagen, T, HM Berger, BM Larsen & R Saksgard (1995) Monitoring sh stocks in relation to acidi cation in Norwegian watersheds Water Air and Soil Pollution, 85 (2), 641 646 Hewitt, LM, L Tremblay, GJ van der Kraak, KR Solomon & MR Servos (1998) Identi cation of the lampricide 3-tri uoromethyl-4-nitrophenol as an agonist for the rainbow trout estrogen receptor Environmental Toxicology and Chemistry, 17, 425 432 Hoffman, ER & S Fisher (1994) Comparison of a eld and laboratory-derived population of Chironomus riparius (Diptera, Chironomidae) biochemical and tness evidence for population divergence Journal of Economic Entomology, 87 (2), 320 325 Horst, TJ (1977) Use of the Leslie matrix for assessing environmental impact with an example for a sh population.

A signi cant decrease in the contents of smoke carbonyl compounds caused by reactions with gelatine was shown by Ziemba (1969) High loss of available lysine in sausage casings due to smoking was found by Ruiter.

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