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Biology and biochemistry of nitrogen xation The overall reaction for biological nitrogen xation is: 8H+ + 8 e + N2 2NH3 + H2, requiring an input of ~16 to 24 ATP. The enzyme system also fortuitously acts on the triple bonded molecule acetylene HCcCH, creating ethene H2CbCH2. Two distinctive nitrogenase enzyme systems have been described in prokaryotes. 1 The most commonly distributed system (in free-living aerobic and anaerobic prokaryotes, some associated as symbionts with root structures in plants) delivers ATP and reducing power (as a reduced avodoxin protein) to a multistep electron transport chain that ends with a Fe-Mo protein (dinitrogenase) that converts a single molecule of N2 to two molecules of NH3. Hydrogen gas is also produced (Figure 1). At least two additional structural variants in the dinitrogenase enzyme have been characterized one with a Fe-V metal cluster and another with only Fe. The reaction is poisoned by molecular oxygen, and aerobic prokaryotes (e.g., Cyanobacteria, Azotobacter, Rhizobium) have evolved clever and effective ways to shield their nitrogenase systems from atmospheric oxygen. 2 Another enzymatic strategy for nitrogen xation has been recently described in Streptomyces thermoautotrophicus (Ribbe et al., 1997). Based on phylogenetic analysis of component proteins, this system appears to be an evolutionary hybrid created by merging enzymes from the above (classic) nitrogenase system with other enzymes derived from the superoxide dismutase oxygen-detoxi cation system. Remarkably, molecular oxygen is a required reactant in the electron-transport chain that delivers reducing power to a molybdenum-containing dinitrogenase enzyme.



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You can also complete the path by clicking the first point you created, but if you do, you create a closed path. Figure 16.9 shows a finished line that contains several curved segments.

+ $ z [ U s ( x )+ u 9 ( z ) ]+ i z [ds(x)+ d,(z)] + ;z[s(z) + s(z)]+ . .. (sea)





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1. Create an object with a dashed line stroke and no fill. I used outlined text with a dashed stroke but no fill. 2. Choose Effect 3D Extrude & Bevel. The Extrude & Bevel Options dialog box opens. 3. Click the Preview check box. This lets you preview the image as you modify it. 4. Set the Extrude depth in points. Alternatively, you can click and drag the slider, change the views by picking an option from Position, or drag the box to a different view. 5. Click OK to see the extruded dashed lines. Figure 16.4 shows the resulting effect.

Both the Go to target field check box and the Specify button present the Specify Field dialog so that you can designate the target field. Their use is interchangeable.

To modify any inserted horizontal rule, simply click it. (If the Property inspector is not already open, you have to double-click the rule.) As a general practice, size your horizontal rules using the percentage option if you are using them to separate items on a full screen. If you are using the horizontal rules to divide items in a specifically sized table column or cell, use the pixel method.

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var j = 0; while (j < 10) {

Photoshop To crop an image in Photoshop, click the Crop tool. Then drag it to select the area you want to crop. When the area is selected, double-click within it. Alternatively, select the portion you want and choose Image Crop from the menu. Fireworks To crop an image in Photoshop, click the Crop tool. Then drag it to select the area you want to crop. When the area is selected, double-click within it. You can also select the part to be cropped, then choose Edit Crop Document from the menu. Painter To crop an image in Painter, click the Crop tool. Then drag it to select the area you want to crop. When the area is selected, click within it (the cursor changes to an animated pair of scissors when it is over the selected area). Paint Shop Pro To crop an image in Paint Shop Pro, click the Crop tool. Then drag it to select the area you want to crop. When the area is selected, double-click anywhere on the image. Alternatively, select the area, then choose Image Crop to Selection from the menu.

Here are some helpful online resources if you get stuck: The 33 official SketchUp website (www.sketchup.com) contains a community forum. This is a great place to ask questions. Many advanced users live on the SketchUcation forums (www.sketchucation.com). This 33 must-visit website is an excellent resource to get help, download custom Ruby Scripts, and learn about current SketchUp trends.

Figure 4-6: You can monitor the status of one or more background refresh operations from this dialog box.

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