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substrate proteins which interact with several GroEL subunits at a time. The unfolded protein consequently dissociates and is released into the cavity where it attempts to fold. In vivo, GroEL requires the co-chaperone GroES which is also a heat shock protein (Hsp 10) and is assembled into a heptamer. This acts as a lid which covers one end of the GroEL cylinder (Figures 6.9 and 6.10). The initial binding of unfolded protein to the cis ring leads to dissociation of GroES from the trans ring. GroES can bind to the cis ring of the GroEL cylinder at the same time as ATP causing the cavity formed by the adjacent (cis) ring of 58 kDa subunits to enlarge relative to the distal (trans) ring by structural effects on the apical domain of the cis ring subunits (Figure 6.10). This forms a hydrophilic structure which is sometimes referred to as the An nsen cage (Figure 6.10). ATP binding to the trans ring of 58 kDa subunits causes release of GroES which opens the cage allowing folded protein either to escape or, if incompletely folded, to undergo another cycle of binding and folding. On each cycle of binding and release of unfolded protein, GroES binds at alternate ends of the



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But how have they done with the renewal side of the online annual fund campaigning The reader is invited to guess at the renewal rate for the 2,000 online donors who ve given from April 2001 to the start of 2004: 0% 10% 40% 60% 90% The answer is ZERO! Did you guess correctly The answer might be a bit surprising, but there is a simple answer for it: the food bank hasn t addressed the full definition of an annual fundraising campaign, which is to not only acquire new donors, but also to renew donors Unfortunately, it has not asked for a second gift from any of the past donors Now, there are a few good reasons for this The first is the fact that wwwcanada helps.





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org is a free donation service that doesn t provide the e-mail addresses to the charity so that it might build a relationship The second reason is that the medium is still very young, and it just isn t thinking of the potential yet The Daily Bread Food Bank does have a direct-mail program that follows a traditional annual repeating structure: (1) a renewal mailing during Easter, (2) a renewal mailing during the summer time, (3) a renewal mailing during the Thanksgiving food drive, and, finally, (4) a year-end holiday mailing The good news is that The Daily Food Bank is about to address this issue in its online annual fund giving It is planning to send out renewal e-mail solicitations at the same time as the direct-mail program.

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After it has added this component to its online program, it will have an online fundraising endeavour that may raise hundreds of thousands of dollars a year at a cost of only a few thousand dollars The final addition of an online renewal schedule will make the food bank s online giving even more efficient..

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