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Liquid cultures of puri ed CD34+ (HPC) with a preestablished MSC feeder layer result in the expansion of CD34+/38+ HPC and CD34+/38 hematopoietic SCs and support the growth of mature hematopoietic total nucleated cell (TNC) progeny (Figure 9). Genetic Stability and Potential Tumorigenicity Genetic analysis of human MSCs is not well established. The signi cance of standard metaphase chromosome G banding is limited due to the low number of metaphases recovered during standard analyses. Advances in multicolor uorescence in situ hybridization (FISH) and highresolution array-based techniques may also soon be translated into practicable diagnostic tools in relation to CBMP safety in regenerative medicine [55]. Genetic instability can occur as a rare event after extended culture of mouse and human MSCs in FBS-supplemented medium [56, 57]. To test for potential in vivo tumor formation, MSCs derived from short-term clinical-scale expansions in FBSor HPL-supplemented media were injected into immunocompromised athymic nude mice subcutaneously. Putative tumor formation was evaluated by histological analyses three months after injection of 2 106 and 2 104 MSCs and compared to controls that were injected 48 h prior to euthanasia. A primary cell deposit was visible immediately and 48 h after injection and MSCs could be recovered by conventional microscopic evaluation. However, none of 12 animals tested developed a macroscopic or microscopic detectable tumor over the 90-day observation period [23]. In this situation, genetic testing may be encouraged for prospective data acqui-



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In the table, the columns headed P show the ratings from Company P s perspective, and those headed C show ratings from the customer s It should be noted that the simple counts of ratings in Table 116 do not necessarily provide a complete guide to.

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Most vendors provide their own AT plan, but usually the plan is not thorough enough; it is a template that is not customized to the speci c healthcare institution s needs. The following sections describe some of the steps in designing and developing a robust AT that can be used for the nal turnover of PACS in the clinical environment. 18.6.1 Resources and Tools One of the rst steps is to identify resources that will be involved in both AT design and implementation. Usually the PACS manufacturer will supply one resource person to be involved in the AT, and it is typically the vendor s PACS project manager. However, it is necessary for some in-house personnel to be involved in the AT. For example, the PACS system administrator can gain knowledge or even provide knowledge about the new PACS during AT development and implementation. A technologist representative can assist in the acquisition portion of end-to-end testing of PACS. Finally, a RIS support person can assist in the RIS portion of the endto-end testing of PACS. As for the AT tools, the vendor usually provides a system acceptance document/checklist. However, it is usually a boiler plate document and not customized to the client s needs. Therefore it is important to ensure that all acceptance criteria and test development are client-driven. The checklist should include the following: 1. 2. 3. 4. Test description Pass/fail criteria and comments Who performed the test Any performance times

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By the way, you can also initialize properties at the time that you declare them, much like you can with variables:

You can insert data from a spreadsheet or text document and maintain a link to the original file, so that if the original file changes, the inserted data is updated as well, very similar to xrefs. For example, you could use this feature to place a schedule of doors and windows in an architectural drawing or a bill of materials in a mechanical drawing. You have two ways to link data. First, you can link data using INSERTOBJ, as described earlier in this chapter. Second, you can use the Clipboard, by following these steps: 1. Open the source application, select the data, and copy it to the Clipboard. (Click Copy on the Standard toolbar or choose Edit Copy.) 2. If your drawing is open, choose its button on the task bar. If not, open it. 3. In your drawing, choose Edit Paste Special. 4. In the Paste Special dialog box, choose Paste Link. Click OK. When you create a link, you don t have all of the format options that you do when you simply paste. You can create a link in the source application s format only.

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Unlike most other procedures, the minimal model of glucose kinetics is based on a physiological de nition of insulin sensitivity from which quantitative estimates can be obtained. The model was rst developed to interpret the results of intravenous glucose tolerance tests (Bergman et al., 1979), but more recently has been adapted

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