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three primitives can only be connected to each other in three ways A match state Mi is connected to insert state Ii , delete state Di+1 and match state Mi+1 An insert state Ii is connected to itself, delete state Di+1 and match state Mi+1 A delete state Di is always connected to insert state Ii , delete state Di+1 and match state M i+1 1 A simple HMM for the four sequences above can now be constructed First, assume that the four sequences have been initially aligned (Table 42) The alignment results in a consensus sequence AGC (columns 2, 3 and 5) One heuristic in building an HMM from scratch is to start with as many match states as there are symbols in the consensus sequence, which in this case is three.



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Another heuristic is to calculate the average length of the training sequences and to start with as many match states In the example here, the length of the consensus sequence is used and therefore three match states are chosen Once the consensus sequence AGC has been found, the empty HMM can be constructed consisting of three match (M) states (one for each symbol of the consensus), four insert (I) states and three delete (D) states (Figure 45(a)), following the rules for structuring HMMs The consensus symbols can then be inserted into each match state The rst sequence AGTC is then entered (Figure 45(b)) The path followed by AGTC is given by thick arrows, with the symbols inserted into the appropriate states.

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After begin , A is matched against M1, G is matched against M2, and T, since it cannot be matched against M3, requires a transition to I2, followed by a match of C against M3 The second sequence CAGC is then entered (Figure 45(c)) Since the rst symbol C cannot be matched against M1, a transition to I0 is required, followed by three matches of A, G and C against M1, M2 and M3, respectively A note is kept of the transitions.

a class is a variable that is associated with the class. However, all instances of the class share that same data, so the data is not really part of any articular object. To see why these might be used, suppose&at you want to build a system that has two FastCRC16 objects in it:

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where an is chosen so that qn (0) qn(1) = 1. Make a tentative decision: Set 2n = 1 if q n ( l ) > 0.5, else set = 0. If A t = 0, then Stop. Otherwise, if #iterations c L , loop to Horizontal Step Otherwise, declare a decoding failure and Stop.

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Once a sound resource has been attached or loaded into a Sound object, you can control playback of the sound with the methods described in this section.

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down bugs. The additional overhead of hand-coding, testing, and debugging business-tier functionality in each new Action eats into the schedule. Changes in the business tier often trigger wholesale modifications to Action subclasses because their implementations are tightly coupled. The general form of this pitfall is to find business-tier code sprinkled liberally through a number of Action classes. For example, developers implementing an EJB-based application might, in the absence of clear guidelines to the contrary, code home interface lookups and direct calls to Session Beans or even to Entity Beans in their Actions, as in this execute() method:

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The degree of r ( x ) is 5 d - 2. This remainder can be computed using conventional LFSR hardware that might be used for the encoding operation, with computational complexity Ob).

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stead on reducing the length of the equipment setups, thereby eliminating the need to create long production runs to reduce per-unit costs To do this, a videotape is made of a typical setup, and then a team of industrial engineers and machine users peruse the tape, spotting and gradually eliminating steps that contribute to a lengthy setup It is not unusual, after several iterations, to achieve setup times of minutes or seconds, when the previous setup times were well into the hours By taking this step, a company reduces the amount of work-in-process, while also shrinking the number of products that can be produced before defects are identified and fixed, thereby reducing scrap costs It is not sufficient to reduce machine setup times, because there are still problems with machines not being coordinated properly, so that there is a smooth and streamlined flow of parts from machine to machine.

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