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1. G. A. Olah, G. K. S. Prakash, and J. Sommer, Superacids, Wiley-Interscience, New York, 1985, 5, p. 243. 2. G. A. Olah and G. K. S. Prakash, in The Chemistry ofAlkanes and Cycloalkanes, S. Patai and Z. Rappoport, eds., Wiley, Chichester, 1992, 13, p. 624. 3. D. Alker, D. H. R. Barton, R. H. Hesse, J. Lister-James, R. E. Markwell, M. M. Pechet, S. Rozen, T. Takeshita, and H. T. Toh, New J. Chem. 4, 239 (1980). 4. C. Gal and S. Rozen, Tetrahedron Lett. 25, 449 (1984). 5. S. Rozen and C. Gal, J. Org. Chem. 52, 2769 (1987). 6. G. A. Olah, N. Hartz, G. Rasul, Q. Wang, G. K. S. Prakash, J. Casanova, and K. D. Christie, J. Am. Chem. Soc. 116,5671 (1994). 7. G. A. Olah, R. Renner, P. Schilling, and Y. K. Mo, J. Am. Chem. Soc. 95,7686 (1973). 8. G. A. Olah and P. Schilling, J. Am. Chem. Soc. 95, 7680 (1973). 9. G. A. Olah, Acc. Chem. Res. 20, 422 (1987). 10. G. A. Olah, B. Gupta, M. Farina, J. D. Felberg, W. M. Ip, A. Husain, R. Karpeles, K. Lammertsma, A. K. Melhotra, and N. J. Trivedi, J. Am. Chem. Soc. 107,7097 (1985). 11. G. A. Olah, U. S. Patent 4,523,040 (1985). 12. 1. Bucsi and G. A. Olah, Catal. Lett. 16,27 (1992). 13. P. Batamack, 1. Bucsi,



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The basic structural information available from intrinsic uorescence spectroscopy of biological macromolecule structures is quite limited compared with information available from even UV visible or even CD spectroscopy and is largely limited to globular proteins. One of the main reasons for this is that only proteins and nucleic acids possess substantial numbers of chromophores that are potentially capable of absorbing photons leading to uorescence emission. However, not every chromophore is simultaneously a good uorophore (i.e. chromophore capable of generating uorescence emission). Indeed, the nucleic acid bases are fundamentally poor uorophores. In proteins, only tryptophan amino-acid residues are substantial uorophores out of the three main aromatic amino-acid chromophores. Moreover, values of F are low in water, though values of R are short, hence generally limiting uorescence utility even further (Table 4.1). In spite of these limitations, uorescence spectroscopy of proteins involving intrinsic tryptophan residue uorescence (excitation 295 nm) can be a useful monitor of conformational changes in globular proteins in a way similar to UV visible spectroscopy of tryptophan residues in globular proteins. If the local environment of a tryptophan residue is changed from hydrophilic (or polar) to hydrophobic, then this leads to an increase in uorescence emission ( * ) transition energies associated with a decrease in transition wavelength (blue-shift) (typically 350 330 nm). Since the interior of such a protein may be regarded as hydrophobic whilst the exterior is hydrophilic, then we can generalise to say that interior buried residues are blue-shifted compared with solvent-exposed residues. Furthermore, interior buried residues will not be subject to internal conversion problems caused by random collisions with water molecules, or to collisional deactivation with quenching molecules. Therefore, values of F should increase substantially according to Equation (4.21), resulting in signi cantly enhanced uorescence emission intensities. Both characteristics ensure that

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