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These cells are rare constituents within any tissue, dif cult to isolate, and in many cases intractable to expansion in culture Rodents and other animal models, in addition to providing a copious source of genetically homogeneous stem cells, also provide excellent physiological microenvironments to test for their function In contrast, access to live tissue specimens from humans can be dif cult and laborious to obtain, and typically the genetic heritage and history of the specimen is unknown Perhaps most importantly, short of clinical trials, it is exceedingly dif cult to test the function of a putatively isolated stem cell from a human in its native environment, and using immunologically impaired or humanized rodents, while useful, does not always work or present an accurate picture of what happens in humans.

Therefore, to facilitate the study of human stem cell biology, one can make the argument that physiologically functional culture models are essential Because traditional two-dimensional (2D) cell culture has many artifacts, and the conditions have been compared to a wound healing state [Bissell, 1981], its usefulness is often and understandably challenged by some stem cell biologists [Joseph and Morrison, 2005] Indeed, culturing primary human cells is dif cult, as they are prone to selection and senescence Nearly four decades of novel work and methods adapted from other elds of study have addressed and solved many of these problems for the study of mammary gland stem cells Using three-dimensional (3D) laminin-rich extracellular matrices (lrECM) and broblast feeder layers or other 3D assays, investigators have successfully recapitulated many key components of stem cell niches in culture models that are more physiologically relevant.

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1. Open CustomButtonSkin.mxml in Source mode. 2. Locate the Label component toward the bottom of the file with an id of displayLabel. 3. Set the Label component s color to purple and its fontSize to 18:

In addition to some successful 2D culture approaches, methods for passaging primary mammary stem cells as mammospheres, which were modeled after methods for maintaining neural stem cells in culture, have been described [Dontu et al, 2003] Methods for immortalizing different subsets of mammary epithelia that seem to allow cells to retain an acceptable normal phenotype, while still bypassing senescence in 2D culture, represent an invaluable advance in this eld Such techniques have fostered more detailed functional and morphological analysis in 3D cultures and in murine implants [Wazer et al, 1995; Gudjonsson et al, 2002b; Kuperwasser et al, 2004] Together, this panoply of assays has allowed for a highly tractable experimental model system with which to unravel the mysteries of mammary stem cell biology This.

And the same fundamental human problem exists with e-cash as with debit cards. When people use credit cards, they can wait to pay them off. With e-cash, they lose their money on the spot. We re not holding our breath on this one.

4-4.2

1. Right-click the QAT and select Customize Quick Access Toolbar. The Customize panel of the Excel Options dialog box appears. 2. In the Choose Commands From drop-down list, select Commands Not In The Ribbon. 3. In the list box on the left, select Form 4. Click the Add button to add the selected command to your QAT. 5. Click OK to close the Excel Options dialog box.

Adding OLE objects to a layout (Windows only)

Video players, such as the one illustrated in Figure 13.5, are becoming more and more popular as the Internet continues to get faster. The idea behind this section is to develop a video player that is interfaced with PHP to load in the video list, as well as keep track of the play count. Flash already ships with a pretty versatile video player component, but you can still expand upon it, which is exactly what this section will help you achieve. The application is constructed of a VideoPlayer and ComboBox component that has already been placed on the Stage. The start file {START_FILE} is provided so you can focus on the development and can quickly test as you go.

27: Removing the 4GB Microdrive from the Apple iPod mini . . . . . . . . . . . . . . . . . . . . . . . . . . 459

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